Installing navigate and navigate MMcore

[jcknowles]

I am not a programmer so have been tryin to step through the instructions.

Ive installed miniconda
Got this running.
Installed Navigate.
Using C:\users\lighsheet folder
Downloaded (is this the same as cloning?) MMCore zip file and unzipped it to C:\users\lighsheet\navigate-mmcore-plugin-develop folder
Configured the windows environment

If I run navigate as navigate -sh it starts

If I run just navigate i get the following errors

vvvvvvvvvvvvvvvvvvvvvvvvvvv Child Process Traceback vvvvvvvvvvvvvvvvvvvvvvvvvvv
Traceback (most recent call last):
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\site-packages\navigate\model\concurrency\concurrency_tools.py", line 501, in child_loop
obj = initializer(*initargs, **initkwargs)
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\site-packages\navigate\model\model.py", line 115, in init
devices_dict = load_devices(
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\site-packages\navigate\model\device_startup_functions.py", line 1393, in load_devices
camera = load_camera_connection(configuration, id, is_synthetic)
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\site-packages\navigate\model\device_startup_functions.py", line 172, in load_camera_connection
HamamatsuController = importlib.import_module(
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\importlib_init.py", line 127, in import_module
return _bootstrap._gcd_import(name[level:], package, level)
File "", line 1030, in _gcd_import
File "", line 1007, in _find_and_load
File "", line 986, in _find_and_load_unlocked
File "", line 680, in _load_unlocked
File "", line 850, in exec_module
File "", line 228, in _call_with_frames_removed
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\site-packages\navigate\model\devices\APIs\hamamatsu\HamamatsuAPI.py", line 38, in
_dll = windll.LoadLibrary("dcamapi.dll")
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\ctypes_init.py", line 460, in LoadLibrary
return self.dlltype(name)
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\ctypes_init.py", line 382, in init
self._handle = _dlopen(self._name, mode)
FileNotFoundError: Could not find module 'dcamapi.dll' (or one of its dependencies). Try using the full path with constructor syntax.
^^^^^^^^^^^^^^^^^^^^^^^^^^^ Child Process Traceback ^^^^^^^^^^^^^^^^^^^^^^^^^^^
vvvvvvvvvvvvvvvvvvvvvvvvvvv Main Process Traceback vvvvvvvvvvvvvvvvvvvvvvvvvvvv
Traceback (most recent call last):
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\runpy.py", line 197, in _run_module_as_main
return _run_code(code, main_globals, None,
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\runpy.py", line 87, in run_code
exec(code, run_globals)
File "C:\Users\Lightsheet\miniconda3\envs\navigate\Scripts\navigate.exe_main.py", line 7, in
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\site-packages\navigate\main.py", line 113, in main
Controller(
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\site-packages\navigate\controller\controller.py", line 195, in init
self.model = ObjectInSubprocess(
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\site-packages\navigate\model\concurrency\concurrency_tools.py", line 383, in init
assert _get_response(self) == "Successfully initialized"
File "C:\Users\Lightsheet\miniconda3\envs\navigate\lib\site-packages\navigate\model\concurrency\concurrency_tools.py", line 438, in _get_response
raise resp
FileNotFoundError: Could not find module 'dcamapi.dll' (or one of its dependencies). Try using the full path with constructor syntax.

[AdvancedImagingUTSW]

Thanks for not hesitating to reach out. Could you also share with us your configuration file from your .navigate folder? I assume navigate opens if you give the command navigate -sh?

At first glance, it appears that you need to install the Hamamatsu drivers. You should make sure that you are able to open your camera using their software.

https://www.hamamatsu.com/us/en/product/cameras/software/driver-software.html

Let me know how it goes and we can connect via Zoom to get things up and working.

Best,
Kevin

[jcknowles]

Many thanks for the rapid reply! Maybe before getting further I should explain what we are trying to do and if navigate could do this We built an openspim lightsheet with an omicron laser, Picard stage and PCO camera We have found the openspim software crashes a lot so were trying to see if there an alternative and found navigate. Would it be able to control the above hardware or not? Thanks Jonathan [signature_3768372275]<http://www.ucl.ac.uk/eastman> Professor Jonathan Knowles, BSc, PhD, FIMMM, FRSC, CEng, FAIMBE Professor of Biomaterials Science Division of Biomaterials and Tissue Engineering UCL Eastman Dental Institute Royal Free Hospital, Rowland Hill Street, London, NW3 2PF, UK Tel: +44 (0)20 8016 7699 My Profile<https://profiles.ucl.ac.uk/5968-jonathan-knowles/about> Editor in Chief: Journal of Biomaterials Applications<https://journals.sagepub.com/home/jba> Editor in Chief: Journal of Tissue Engineering<https://journals.sagepub.com/home/tej> To stay up to date with the latest Institute news: visit the website<http://www.ucl.ac.uk/eastman> | sign up for emails<http://www.ucl.ac.uk/eastman/about/sign-up-for-emails> | follow us on Twitter<http://www.twitter.com/ucleastman> From: Kevin M. Dean ***@***.***> Date: Monday, 25 November 2024 at 12:23 To: TheDeanLab/navigate ***@***.***> Cc: Knowles, Jonathan ***@***.***>, Author ***@***.***> Subject: Re: [TheDeanLab/navigate] Installing navigate and navigate MMcore (Issue #1038) ⚠ Caution: External sender Thanks for not hesitating to reach out. Could you also share with us your configuration file from your .navigate folder? I assume navigate opens if you give the command navigate -sh? At first glance, it appears that you need to install the Hamamatsu drivers. You should make sure that you are able to open your camera using their software. https://www.hamamatsu.com/us/en/product/cameras/software/driver-software.html Let me know how it goes and we can connect via Zoom to get things up and working. Best, Kevin — Reply to this email directly, view it on GitHub<#1038 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AAOVD5K2U4RWFSFNSO7LKHT2CMJENAVCNFSM6AAAAABSN3ADHWVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDIOJXHA3TIMJQGE>. You are receiving this because you authored the thread.Message ID: ***@***.***>

[AdvancedImagingUTSW]

Hello Johnathan,

I just quickly reviewed the parts list for the OpenSPIM, and we can get you most of the way there. It ultimately depends upon your timeline and willingness to purchase another piece of equipment.

Navigate uses National Instruments data acquisition cards to perform all analog and digital triggering of cameras and lasers. It does not seem like the OpenSPIM requires this. Would you happen to have one of these cards lying around? They run around $3k USD (see attached quote from Newark). Additionally, we would have to add support for the PCO camera, but I don't suspect that would be too difficult. The PCO camera has a great API, and we know several labs who routinely control these cameras with their own Python software.

Otherwise, the Picard stage is supported my the MMCore plugin, and @annie-xd-wang would be able to help you with that. And most likely, we could wire up the lasers for analog/digital external control no problem. What exact model of Omicron do you have? We typically use the LightHUB systems...

All the best,
Kevin

Newark[41].pdf

[jcknowles]

Hi Again We do have some NI hardware lying around I will check. We actually trigger our laser via an Arduino which works in Micromanager. Would this work instead? I will check the boxes for NI cards Jonathan [signature_384482827]<http://www.ucl.ac.uk/eastman> Professor Jonathan Knowles, BSc, PhD, FIMMM, FRSC, CEng, FAIMBE Professor of Biomaterials Science Division of Biomaterials and Tissue Engineering UCL Eastman Dental Institute Royal Free Hospital, Rowland Hill Street, London, NW3 2PF, UK Tel: +44 (0)20 8016 7699 My Profile<https://profiles.ucl.ac.uk/5968-jonathan-knowles/about> Editor in Chief: Journal of Biomaterials Applications<https://journals.sagepub.com/home/jba> Editor in Chief: Journal of Tissue Engineering<https://journals.sagepub.com/home/tej> To stay up to date with the latest Institute news: visit the website<http://www.ucl.ac.uk/eastman> | sign up for emails<http://www.ucl.ac.uk/eastman/about/sign-up-for-emails> | follow us on Twitter<http://www.twitter.com/ucleastman> From: Kevin M. Dean ***@***.***> Date: Monday, 25 November 2024 at 13:43 To: TheDeanLab/navigate ***@***.***> Cc: Knowles, Jonathan ***@***.***>, Author ***@***.***> Subject: Re: [TheDeanLab/navigate] Installing navigate and navigate MMcore (Issue #1038) ⚠ Caution: External sender Hello Johnathan, I just quickly reviewed the parts list for the OpenSPIM, and we can get you most of the way there. It ultimately depends upon your timeline and willingness to purchase another piece of equipment. Navigate uses National Instruments data acquisition cards to perform all analog and digital triggering of cameras and lasers. It does not seem like the OpenSPIM requires this. Would you happen to have one of these cards lying around? They run around $3k USD (see attached quote from Newark). Additionally, we would have to add support for the PCO camera, but I don't suspect that would be too difficult. The PCO camera has a great API, and we know several labs who routinely control these cameras with their own Python software. Otherwise, the Picard stage is supported my the MMCore plugin, and @annie-xd-wang<https://github.com/annie-xd-wang> would be able to help you with that. And most likely, we could wire up the lasers for analog/digital external control no problem. What exact model of Omicron do you have? We typically use the LightHUB systems... All the best, Kevin Newark[41].pdf<https://github.com/user-attachments/files/17903695/Newark.41.pdf> — Reply to this email directly, view it on GitHub<#1038 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AAOVD5I7B7TJ34RTBS7HDYL2CMSNPAVCNFSM6AAAAABSN3ADHWVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDIOJYGA2TMOJSGU>. You are receiving this because you authored the thread.Message ID: ***@***.***>

[jcknowles]

Weve got a USB I/O unit. The nearest PCIe card we have is a 6320 https://www.newark.com/ni/781043-01/pcie-6320-multifun-i-o-device/dp/13AJ3954 Jonathan [signature_1673973874]<http://www.ucl.ac.uk/eastman> Professor Jonathan Knowles, BSc, PhD, FIMMM, FRSC, CEng, FAIMBE Professor of Biomaterials Science Division of Biomaterials and Tissue Engineering UCL Eastman Dental Institute Royal Free Hospital, Rowland Hill Street, London, NW3 2PF, UK Tel: +44 (0)20 8016 7699 My Profile<https://profiles.ucl.ac.uk/5968-jonathan-knowles/about> Editor in Chief: Journal of Biomaterials Applications<https://journals.sagepub.com/home/jba> Editor in Chief: Journal of Tissue Engineering<https://journals.sagepub.com/home/tej> To stay up to date with the latest Institute news: visit the website<http://www.ucl.ac.uk/eastman> | sign up for emails<http://www.ucl.ac.uk/eastman/about/sign-up-for-emails> | follow us on Twitter<http://www.twitter.com/ucleastman> From: Kevin M. Dean ***@***.***> Date: Monday, 25 November 2024 at 13:43 To: TheDeanLab/navigate ***@***.***> Cc: Knowles, Jonathan ***@***.***>, Author ***@***.***> Subject: Re: [TheDeanLab/navigate] Installing navigate and navigate MMcore (Issue #1038) ⚠ Caution: External sender Hello Johnathan, I just quickly reviewed the parts list for the OpenSPIM, and we can get you most of the way there. It ultimately depends upon your timeline and willingness to purchase another piece of equipment. Navigate uses National Instruments data acquisition cards to perform all analog and digital triggering of cameras and lasers. It does not seem like the OpenSPIM requires this. Would you happen to have one of these cards lying around? They run around $3k USD (see attached quote from Newark). Additionally, we would have to add support for the PCO camera, but I don't suspect that would be too difficult. The PCO camera has a great API, and we know several labs who routinely control these cameras with their own Python software. Otherwise, the Picard stage is supported my the MMCore plugin, and @annie-xd-wang<https://github.com/annie-xd-wang> would be able to help you with that. And most likely, we could wire up the lasers for analog/digital external control no problem. What exact model of Omicron do you have? We typically use the LightHUB systems... All the best, Kevin Newark[41].pdf<https://github.com/user-attachments/files/17903695/Newark.41.pdf> — Reply to this email directly, view it on GitHub<#1038 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AAOVD5I7B7TJ34RTBS7HDYL2CMSNPAVCNFSM6AAAAABSN3ADHWVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDIOJYGA2TMOJSGU>. You are receiving this because you authored the thread.Message ID: ***@***.***>

[AdvancedImagingUTSW]

The PCIe card would work for triggering cameras, shutters, and digital modulation of lasers. It does not have analog outputs, so laser intensity would have to be set using the Omicron software.

There are no galvos, right? It is a sample scanning system with a static light-sheet?

[jcknowles]

Hi The system is just a Move sample/turn laser on/take image/turn laser off/move to next position. As I mentioned the laser control is currently done via the Arduino using a TTL output. We only have one wavelength at the moment Thanks Jonathan [signature_3276897447]<http://www.ucl.ac.uk/eastman> Professor Jonathan Knowles, BSc, PhD, FIMMM, FRSC, CEng, FAIMBE Professor of Biomaterials Science Division of Biomaterials and Tissue Engineering UCL Eastman Dental Institute Royal Free Hospital, Rowland Hill Street, London, NW3 2PF, UK Tel: +44 (0)20 8016 7699 My Profile<https://profiles.ucl.ac.uk/5968-jonathan-knowles/about> Editor in Chief: Journal of Biomaterials Applications<https://journals.sagepub.com/home/jba> Editor in Chief: Journal of Tissue Engineering<https://journals.sagepub.com/home/tej> To stay up to date with the latest Institute news: visit the website<http://www.ucl.ac.uk/eastman> | sign up for emails<http://www.ucl.ac.uk/eastman/about/sign-up-for-emails> | follow us on Twitter<http://www.twitter.com/ucleastman> From: Kevin M. Dean ***@***.***> Date: Monday, 25 November 2024 at 14:53 To: TheDeanLab/navigate ***@***.***> Cc: Knowles, Jonathan ***@***.***>, Author ***@***.***> Subject: Re: [TheDeanLab/navigate] Installing navigate and navigate MMcore (Issue #1038) ⚠ Caution: External sender The PCIe card would work for triggering cameras, shutters, and digital modulation of lasers. It does not have analog outputs, so laser intensity would have to be set using the Omicron software. There are no galvos, right? It is a sample scanning system with a static light-sheet? image.png (view on web)<https://github.com/user-attachments/assets/9232d58f-e1d3-4c95-99e3-be30c2a510b8> — Reply to this email directly, view it on GitHub<#1038 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AAOVD5IC2QKY3GGM6RIRAL32CM2X7AVCNFSM6AAAAABSN3ADHWVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDIOJYGI2DCMBVG4>. You are receiving this because you authored the thread.Message ID: ***@***.***>

[AdvancedImagingUTSW]

That sounds simple enough. What is the exact model of the PCO camera that you have? That is the last thing holding us up...

Best,
Kevin

[jcknowles]

From memory it’s a pco edge 4.2 usb3. Would that be correct? Am on the way home so can’t check! Professor J.C. Knowles BSc(hons), PhD, FAIMBE, FIMMM, CEng, FRSC, CSci Professor of Biomaterials Science Head of Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, Royal Free Hospital, Rowland Hill Street London NW3 2PF. UK. Telephone +44 020 8016 7699 Mobile 07785 313615 Editor, Journal of Tissue Engineering Editor, Journal of Biomaterials Applications Email ***@***.******@***.***> Eastman website; http://www.ucl.ac.uk<http://www.ucl.ac.uk/>/eastman Personal website; http://www.homepages.ucl.ac.uk/~sfhvjck/ On 25 Nov 2024, at 15:35, Kevin M. Dean ***@***.***> wrote:  ⚠ Caution: External sender That sounds simple enough. What is the exact model of the PCO camera that you have? That is the last thing holding us up... Best, Kevin — Reply to this email directly, view it on GitHub<#1038 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AAOVD5L2S5B35HP7O4Y5KUT2CM7ULAVCNFSM6AAAAABSN3ADHWVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDIOJYGM2DSNJSHE>. You are receiving this because you authored the thread.Message ID: ***@***.***>

[AdvancedImagingUTSW]

Sounds good. Thanks for the information. Before you do anything, the first thing we need to do is evaluate how difficult it will be for us to add software for controlling that PCO camera. Please let us know what your timeline is. It won't be overnight on our side to add new code for controlling the camera.

[jcknowles]

Not in any rush with this thanks Professor J.C. Knowles BSc(hons), PhD, FAIMBE, FIMMM, CEng, FRSC, CSci Professor of Biomaterials Science Head of Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, Royal Free Hospital, Rowland Hill Street London NW3 2PF. UK. Telephone +44 020 8016 7699 Mobile 07785 313615 Editor, Journal of Tissue Engineering Editor, Journal of Biomaterials Applications Email ***@***.******@***.***> Eastman website; http://www.ucl.ac.uk<http://www.ucl.ac.uk/>/eastman Personal website; http://www.homepages.ucl.ac.uk/~sfhvjck/ On 25 Nov 2024, at 19:02, Kevin M. Dean ***@***.***> wrote:  ⚠ Caution: External sender Sounds good. Thanks for the information. Before you do anything, the first thing we need to do is evaluate how difficult it will be for us to add software for controlling that PCO camera. Please let us know what your timeline is. It won't be overnight on our side to add new code for controlling the camera. — Reply to this email directly, view it on GitHub<#1038 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AAOVD5M65BBKVSWAZJUR3T32CNX57AVCNFSM6AAAAABSN3ADHWVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDIOJYHAYTEOJYGM>. You are receiving this because you authored the thread.Message ID: ***@***.***>

[jcknowles]

Hi I will ask around at UCL and see if we could borrow one of the NI cards. Would this make things easier? For the laser in micromanager, we could control the power via the USB connection and just switched it on and off via the Arduino Thanks Jonathan [signature_2721750378]<http://www.ucl.ac.uk/eastman> Professor Jonathan Knowles, BSc, PhD, FIMMM, FRSC, CEng, FAIMBE Professor of Biomaterials Science Division of Biomaterials and Tissue Engineering UCL Eastman Dental Institute Royal Free Hospital, Rowland Hill Street, London, NW3 2PF, UK Tel: +44 (0)20 8016 7699 My Profile<https://profiles.ucl.ac.uk/5968-jonathan-knowles/about> Editor in Chief: Journal of Biomaterials Applications<https://journals.sagepub.com/home/jba> Editor in Chief: Journal of Tissue Engineering<https://journals.sagepub.com/home/tej> To stay up to date with the latest Institute news: visit the website<http://www.ucl.ac.uk/eastman> | sign up for emails<http://www.ucl.ac.uk/eastman/about/sign-up-for-emails> | follow us on Twitter<http://www.twitter.com/ucleastman> From: Kevin M. Dean ***@***.***> Date: Monday, 25 November 2024 at 19:02 To: TheDeanLab/navigate ***@***.***> Cc: Knowles, Jonathan ***@***.***>, Author ***@***.***> Subject: Re: [TheDeanLab/navigate] Installing navigate and navigate MMcore (Issue #1038) ⚠ Caution: External sender Sounds good. Thanks for the information. Before you do anything, the first thing we need to do is evaluate how difficult it will be for us to add software for controlling that PCO camera. Please let us know what your timeline is. It won't be overnight on our side to add new code for controlling the camera. — Reply to this email directly, view it on GitHub<#1038 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AAOVD5M65BBKVSWAZJUR3T32CNX57AVCNFSM6AAAAABSN3ADHWVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDIOJYHAYTEOJYGM>. You are receiving this because you authored the thread.Message ID: ***@***.***>

[AdvancedImagingUTSW]

Hello Jonathan,

The NI card you have is perfectly fine. We can control the power of the lasers externally (either via MicroManager or with Omicron's software), and use the NI card to trigger the laser and the camera. Of course, you would need a cable and breakout box to connect to your PCIe-based NI card. We would not use the Ardruino. Annie is looking into the PCO camera and we hope to get back you fairly quickly.

Best,
Kevin

[jcknowles]

Thanks for all the help with this. I think we have a breakout box for the board already. Otherwise I will get one. Also I will install the drivers and card. Anything specific software wise needed for the NI card as I know NI supply a lot of packages with their cards. Professor J.C. Knowles BSc(hons), PhD, FAIMBE, FIMMM, CEng, FRSC, CSci Professor of Biomaterials Science Head of Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, Royal Free Hospital, Rowland Hill Street London NW3 2PF. UK. Telephone +44 020 8016 7699 Mobile 07785 313615 Editor, Journal of Tissue Engineering Editor, Journal of Biomaterials Applications Email ***@***.******@***.***> Eastman website; http://www.ucl.ac.uk<http://www.ucl.ac.uk/>/eastman Personal website; http://www.homepages.ucl.ac.uk/~sfhvjck/ On 26 Nov 2024, at 18:13, Kevin M. Dean ***@***.***> wrote:  ⚠ Caution: External sender Hello Jonathan, The NI card you have is perfectly fine. We can control the power of the lasers externally (either via MicroManager or with Omicron's software), and use the NI card to trigger the laser and the camera. Of course, you would need a cable and breakout box to connect to your PCIe-based NI card. We would not use the Ardruino. Annie is looking into the PCO camera and we hope to get back you fairly quickly. Best, Kevin — Reply to this email directly, view it on GitHub<#1038 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AAOVD5NQH4EFQCMPG3DXPL32CS24JAVCNFSM6AAAAABSN3ADHWVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDKMBRGYZDMNRXGE>. You are receiving this because you authored the thread.Message ID: ***@***.***>

[jcknowles]

Hi Ive found a breakout box and cable. Its an NI SCC-68 Jonathan [signature_2240095356]<http://www.ucl.ac.uk/eastman> Professor Jonathan Knowles, BSc, PhD, FIMMM, FRSC, CEng, FAIMBE Professor of Biomaterials Science Division of Biomaterials and Tissue Engineering UCL Eastman Dental Institute Royal Free Hospital, Rowland Hill Street, London, NW3 2PF, UK Tel: +44 (0)20 8016 7699 My Profile<https://profiles.ucl.ac.uk/5968-jonathan-knowles/about> Editor in Chief: Journal of Biomaterials Applications<https://journals.sagepub.com/home/jba> Editor in Chief: Journal of Tissue Engineering<https://journals.sagepub.com/home/tej> To stay up to date with the latest Institute news: visit the website<http://www.ucl.ac.uk/eastman> | sign up for emails<http://www.ucl.ac.uk/eastman/about/sign-up-for-emails> | follow us on Twitter<http://www.twitter.com/ucleastman> From: Kevin M. Dean ***@***.***> Date: Tuesday, 26 November 2024 at 18:13 To: TheDeanLab/navigate ***@***.***> Cc: Knowles, Jonathan ***@***.***>, Author ***@***.***> Subject: Re: [TheDeanLab/navigate] Installing navigate and navigate MMcore (Issue #1038) ⚠ Caution: External sender Hello Jonathan, The NI card you have is perfectly fine. We can control the power of the lasers externally (either via MicroManager or with Omicron's software), and use the NI card to trigger the laser and the camera. Of course, you would need a cable and breakout box to connect to your PCIe-based NI card. We would not use the Ardruino. Annie is looking into the PCO camera and we hope to get back you fairly quickly. Best, Kevin — Reply to this email directly, view it on GitHub<#1038 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AAOVD5NQH4EFQCMPG3DXPL32CS24JAVCNFSM6AAAAABSN3ADHWVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDKMBRGYZDMNRXGE>. You are receiving this because you authored the thread.Message ID: ***@***.***>