This analysis uses data from the NASA study OSD-599 Version 4: Transcriptomic Effects on the Mouse Heart Following 30 Days on the International Space Station (ISS), available at NASA OSDR Repository. It aims to study the effects of microgravity on the heart-brain axis by exploring upregulated and downregulated genes, and performing Gene Set Enrichment Analysis (GSEA) to connect them to corresponding relevant biological pathways.
- Load and clean the dataset
- Filter genes based on adjusted p-value (≤ 0.05)
- Identify and visualise the top 10 upregulated and downregulated genes by log2 fold change
- Examine selected genes of particular interest
- Perform Gene Set Enrichment Analysis using the
GO_Biological_Process_2021dataset
Chen EY, Tan CM, Kou Y, Duan Q, Wang Z, Meirelles GV, Clark NR, Ma'ayan A.
Enrichr: interactive and collaborative HTML5 gene list enrichment analysis tool. BMC Bioinformatics. 2013; 128(14)